Figure 5.
![Figure 5. IL-15 but not IL-21 induces antiapoptotic effects and CLL B-cell proliferation. The antiapoptotic and mitogenic activities of IL-15 are counteracted by IL-21. (A) CD40-activated CLL B cells were cultured in triplicate wells of 96-well plates in the presence of different concentrations of rhIL-15 and/or rhIL-21 for 48 hours and then pulsed with 3H-thymidine (0.037 MBq/well [1 μCi/well]) for an additional 18 hours of culture. Data are expressed as mean ± SD of 3 repeated experiments. (B) Apoptosis was measured by DiOC6 staining of CLL B cells after 72 hours of culture in the presence, or absence of rhIL-21 and/or rhIL-15 (40 ng/mL). Horizontal bar M1 indicates fluorescence intensity corresponding to signals from viable cells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/9/10.1182_blood-2005-09-3535/2/zh80090694950005.jpeg?Expires=1722777124&Signature=AOUoQ4AzZcc2wzflcgDELqYb5R9BhFGGV4zgm-trW8LdfZVXmCZWXvQwPt9BBQ9MxIOpGGTD0-ZC8Lpqys4VBkB7Zo77bqcuiUCaXyPlMB9tkWPoNa6Fw9OQvWyZ0-IKCO7563Cyo~jKpdRCO0-2nd2YyZcjQgvaZQ3zYYFQCwh4ss6mwb1XykY-mt6l~K0czVvpNeOkdmd3VO0z4L0~D3mUhyVH34kf~Cqjo5Uci0Ks~SGy~Mu7UnEAv7NLML5~IWXFsYBAb0uwraJjr8vPOQA9etXEMluRz-Jg814FFTMolLpikecfbTH-fjNpypmxm0I-7hVR1dpOy8exWLGk3g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IL-15 but not IL-21 induces antiapoptotic effects and CLL B-cell proliferation. The antiapoptotic and mitogenic activities of IL-15 are counteracted by IL-21. (A) CD40-activated CLL B cells were cultured in triplicate wells of 96-well plates in the presence of different concentrations of rhIL-15 and/or rhIL-21 for 48 hours and then pulsed with 3H-thymidine (0.037 MBq/well [1 μCi/well]) for an additional 18 hours of culture. Data are expressed as mean ± SD of 3 repeated experiments. (B) Apoptosis was measured by DiOC6 staining of CLL B cells after 72 hours of culture in the presence, or absence of rhIL-21 and/or rhIL-15 (40 ng/mL). Horizontal bar M1 indicates fluorescence intensity corresponding to signals from viable cells.
