Immunologic characterization of the mononuclear cells infiltrating the foci of VEGF-induced neovascularization. Immunofluorescence staining was performed using CD31-, α-SMA–, Flk-1–, CD34-, Sca-1–, F4/80-, CD11b-, and c-Kit–specific antibodies on histologic sections of mouse tibialis anterior muscles perfused in vivo with FITC-lectin. The analysis was performed at 2 weeks after AAV-VEGF transduction. As shown in the 3 small subpanels, red indicates cells positive for the different antibodies; green, whole vasculature stained with FITC-lectin; and blue, nuclei stained with DAPI. The 3 stainings are merged in the large panels.