PKC412 inhibits autophosphorylation of STAT5 in Ba/F3 cells expressing JM point-mutated FLT3 receptors. Mock cells and cells expressing FLT3-WT, FLT3-W51, FLT3-V592A, FLT3-V579A, FLT3-F594L, and FLT3-F590GY591D were starved for 24 hours and treated with 50 nM PKC412 1 hour before cell lysis. Cell lysates were subjected to Western blot analysis using polyclonal anti-phospho-STAT5 antibody. Blots were stripped and reblotted with polyclonal anti-STAT5 antibody.