Expression of cell cycle- and apoptosis-related proteins in tested cell lines cultured with TZD18. (A) BV173, SD1, and Sup B-15 cells were incubated with or without TZD18 (10 or 20 μM, 4 days), and cell lysates were examined for cell cycle- and apoptosis-related proteins by Western blot, as described in the “Materials and methods.” A representative blot of 3 independent experiments is shown. The relative intensity of each band was quantified using Phoretix ID Quantifier software and was expressed as percentage of control. (B) Equal amounts of protein prepared from SD1 cells exposed to TZD18 (20 μM) for different durations were examined for the same cell cycle- and apoptosis-related proteins as in panel A.