Effect of the PPARα/γ ligand TZD18 on the proliferation of tested cell lines. TZD18 inhibits proliferation of tested cell lines in a dose-dependent (A) and a time-dependent (B) manner. Cells (2 × 105/mL) were incubated in the presence of TZD18 at various concentrations for different numbers of days (measured in hours). Cell proliferation was measured by MTT test; results were expressed as a percentage of control (without treatment). Values are mean ± SD of 6 individual experiments. (C) TZD18 alters cell cycle progression. Cells (2 × 105/mL) were incubated in the presence or absence of TZD18 (10 or 20 μM, 3 days), fixed, treated with RNase, and stained for DNA with PI. Cell cycle distribution was determined by FACS analysis. Results represent the percentage of the total cell population. Figure is representative of 3 independent experiments.
