Expression of c-Met on corneal blood vessels but not lymphatic vessels. A rabbit anti–mouse c-Met specific antibody (B) together with a rat anti–mouse Lyve-1 antibody (A) was used for double staining of corneal tissues with implanted growth factors (A-C) or with suture (D-F). Lyve-1–positive signals are in blue and c-Met–positive signals are in red. No overlapping signals were detected between c-Met and Lyve-1 (C,F). Bar = 100 μm. (G) LEC migration stimulated by various concentrations of HGF or VEGF-C). (H) LEC proliferation stimulated by 100 ng HGF or VEGF-C. Numbers represent averages of 3 determinants for proliferation experiments and 4 determinants for chemotactic experiments (± SEM). Images were captured as described in Figure 2.