Stimulation of lymphangiogenesis. At day 14 after pellet implantation, corneal tissues were used for whole mount double staining with antibodies against Lyve-1 (A, E, I, M, and Q) and CD31 (B, F, J, N, and R). Lyve-1–positive signals are in blue color and CD31⁺ signals are in red color. Lyve-1–positive and CD31⁺ signals in the same areas did not show overlapping staining (C, D, G, H, K, L, O, P, S, and T). Panels D, H, L, P, and T represent enlargement of the boxed areas of panels C, G, K, O, and S, respectively. Bar = 100 μm. White arrows point to Lyve-1–positive structures and yellow arrows point to CD31⁺ structures. Images were captured with a Zeiss LSM 510 confocal laser-scanning microscope (Carl Zeiss, Jena, Germany) that includes an Axiovert 100M confocal microscope and a camera, and with Image Browser 5 LSM ver3.2 (Carl Zeiss). Images were taken with Plan Neofluar 10 ×/0.3 NA objective lenses. Figure panels were prepared with Adobe Photoshop 7.0 (Adobe Systems, San Jose, CA).