Splenic iron and measure of hypoferremia in WT and Tlr4^Lps-d mice upon systemic infection. (A) Iron staining of splenic sections by Perl blue method 24 hour after intraperitoneal challenge with P aeruginosa (PA) or PBS control. (B) Serum iron measurement in WT and Tlr4^Lps-d Tlr4^Lps-d challenged with PA. (C) Hepcidin mRNA levels in WT and Tlr4^Lps-d macrophages upon exposure to iron in the form of diferric transferrin.