Figure 5.
Post-BMT splenic T-lineage progenitors are most efficiently derived from Flt3+ LSK BM cells. Progenitor populations were sorted from Ly5SJL BM and transplanted into lethally irradiated B6 recipient mice together with 105 B6 BM cells to ensure survival. The following populations were assessed: Flt3– LSK cells, Flt3+ LSK cells, and CLPs (Lin–IL-7Rα+AA4.1+Sca-1loc-Kitlo).47,48 The number of cells injected was as follows: Flt3+ LSK, 500; Flt3– LSK, 250; CLPs, 750. These numbers were chosen to be close to or higher than the respective number of each progenitor subset in 105 BM cells. (A) Donor versus host Ly5 staining of spleen cell suspensions at day 16 after BMT. The high percentage of Ly5– cells is due to the presence of erythroid progenitors. (B) Myeloid (Gr-1/CD11b+) and B-lineage (CD19+) progeny of the sorted B6.Ly5SJL progenitor subsets. (C) Generation of T-lineage progenitors as defined in Figure 2. (D) Relative potency Flt3– LSK, Flt3+ LSK cells, and CLPs in the generation of SpT cells. Each triangle represents an individual recipient (n = 6). The mean is shown as a horizontal bar. The difference between each progenitor subset was statistically significant (P < .01, Student t test).

Post-BMT splenic T-lineage progenitors are most efficiently derived from Flt3+ LSK BM cells. Progenitor populations were sorted from Ly5SJL BM and transplanted into lethally irradiated B6 recipient mice together with 105 B6 BM cells to ensure survival. The following populations were assessed: Flt3 LSK cells, Flt3+ LSK cells, and CLPs (LinIL-7Rα+AA4.1+Sca-1loc-Kitlo).47,48  The number of cells injected was as follows: Flt3+ LSK, 500; Flt3 LSK, 250; CLPs, 750. These numbers were chosen to be close to or higher than the respective number of each progenitor subset in 105 BM cells. (A) Donor versus host Ly5 staining of spleen cell suspensions at day 16 after BMT. The high percentage of Ly5 cells is due to the presence of erythroid progenitors. (B) Myeloid (Gr-1/CD11b+) and B-lineage (CD19+) progeny of the sorted B6.Ly5SJL progenitor subsets. (C) Generation of T-lineage progenitors as defined in Figure 2. (D) Relative potency Flt3 LSK, Flt3+ LSK cells, and CLPs in the generation of SpT cells. Each triangle represents an individual recipient (n = 6). The mean is shown as a horizontal bar. The difference between each progenitor subset was statistically significant (P < .01, Student t test).

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