Figure 4.
Tissue distribution and extent of differentiation of post-BMT T-lineage progenitors. B6 mice were lethally irradiated and reconstituted with Ly5SJL BM cells. Three weeks later, cell suspensions from the spleen, MLNs, inguinal lymph nodes, BM, liver, and thymus were assessed by flow cytometry for the presence of donor-derived cells with phenotypic characteristics of T-lineage progenitors (SpT cells, as defined in Figure 2). The 3-week time point was chosen to evaluate the overall extent of prethymic differentiation. Data are representative of 2 experiments. Numbers indicate the percentage of cells in the boxed region. (A) Presence of donor-derived Lin–Thy1.2+ pre-T cells in the spleen, lymph nodes, and thymus. (B) Absence of donor-derived SpT cells in the BM and liver. (C) Generation of donor-derived CD4+CD8+ DP cells in the MLNs, but not in other lymph nodes or in the spleen after BMT.

Tissue distribution and extent of differentiation of post-BMT T-lineage progenitors. B6 mice were lethally irradiated and reconstituted with Ly5SJL BM cells. Three weeks later, cell suspensions from the spleen, MLNs, inguinal lymph nodes, BM, liver, and thymus were assessed by flow cytometry for the presence of donor-derived cells with phenotypic characteristics of T-lineage progenitors (SpT cells, as defined in Figure 2). The 3-week time point was chosen to evaluate the overall extent of prethymic differentiation. Data are representative of 2 experiments. Numbers indicate the percentage of cells in the boxed region. (A) Presence of donor-derived LinThy1.2+ pre-T cells in the spleen, lymph nodes, and thymus. (B) Absence of donor-derived SpT cells in the BM and liver. (C) Generation of donor-derived CD4+CD8+ DP cells in the MLNs, but not in other lymph nodes or in the spleen after BMT.

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