Adhesion of mouse BM Lin^– Sca1⁺c-kit⁺ (LSK) cells to laminins and fibronectin and inhibition cell adhesion to laminins by antibodies against integrin α6 and β1 chain. (A) Adhesion of LSK cells to fibronectin (Fn), laminin-1 (LM-1), laminin-8 (LM-8), and laminin-10/11 (LM-10/11). PBS indicates cell adhesion to wells coated with PBS instead of proteins. The proteins were used at 30 μg/mL. □ indicates cell adhesion without treatment with TPA; ▪, cell adhesion after treatment with TPA. Asterisks indicate significant differences compared with cell adhesion on PBS-coated plates: *P < .05; **P < .01; ***P < .001. Results (mean ± SD) are from 2 experiments performed in triplicate. (B-D) Adhesion of LSK cells to wells coated with laminin-8 (30 μg/mL; B), laminin-10/11 (10 μg/mL; C), and laminin-10 (10 μg/mL; D) in the presence of antibodies GoH3 against integrin α6 chain (α6), Ha2/5 against integrin β1 chain (β1), or control rat or hamster monoclonal antibodies (rIgG and hIgM, respectively). The results are shown as percent cell adhesion of adhesion in the presence of the rIgG control antibody. The antibodies against integrin α6 and β1 chains significantly reduced cell adhesion to laminins, compared with respective isotype control antibodies. **P < .01; ***P < .001. Data represent mean ± SD of 2 experiments performed in triplicate.