Effect of inhibition of deposition of perfused fibronectin on platelet thrombus formation under shear conditions. A suspension of platelets and red blood cells was premixed with 100 nM FITC-fibronectin (A) or unlabeled fibronectin (B-C) in the presence or absence of 1 μM FUD or 70K fragment and perfused through a flow chamber opposed to a coverslip or culture dish coated with fibrin or fibronectin-fibrin for 5 minutes at a shear rate of 1250 s^–1. (A) Coverslips were taken out of the chamber, and microscopy was performed as described in Figure 1. The small and large pictures were taken with × 10 (bar = 100 μm) and × 100 objectives (bar = 10 μm), respectively. (B-C) Thrombus volumes and platelet numbers were measured as described in Table 1. Values represent the mean ± SD (n = 3 experiments).