GILZ production by DCs.GILZ gene expression was determined by real-time RT-PCR. (A) DEX (♦), IL-10 (□), TGFβ (▴), or medium (○) were added to MoDC cultures on days 5 and 7, and CD40L was added on day 7. (B) DEX was added to MoDCs on day 5, cells were washed on day 7, and DEX, CD40L, or medium were added. (C) DEX (), IL-10 (), or medium (○) were added on day 9 of CD34-DC cultures, and CD40L was added on day 10. (D) GILZ expression (bold curves) in untreated MoDCs and in MoDCs treated for 24 hours with DEX, DEX + control siRNA (siC), or DEX + GILZ siRNA (siGILZ) was determined by flow cytometry. Tinted histograms correspond to the control antibody. Means (± SEM) are shown in panels A-C, and a typical experiment, in panel D. n = 5 for panels A and C, and n = 3 for panels B and D.