PVR and Nectin-2 expression on monocytes, iDCs, and mDCs. Monocytes (gated on PBMCs by physical parameters in FSC/SSC dot plot) or cultured iDCs or mDCs (obtained using the indicated culture conditions) were analyzed by flow cytometry with mAbs to the indicated molecules. White profiles represent isotypic negative controls. Numbers indicate the mean fluorescence intensity (MFI).