Spatial distribution of human hematopoietic cells in the murine BM compartment. Representative pictures of bone specimens from NOD/SCID mice at 10 weeks after IBMT of 2 × 105 CBCD34 cells are shown. Slides were stained with either anti-CD34, anti-CD15, or anti-glycophorin A antibody, and cells expressing the respective antigens were distinguished by brown reactive products of DAB on immunohistochemistry. CD34+ cells are localized to the endosteal region (A; black arrowheads), whereas CD15+ (B) and GlyA+ cells (C) are distributed away from the endosteum. Vertical lines demarcate 12 cells of the endosteum. Bars represent 100 μm. Images were obtained using an Olympus AX80 microscope and a 20 ×/0.7 NA UPlanApo objective lens. Images were captured using a DP50 digital camera fitted with Viewfinder Lite (all from Olympus, Tokyo, Japan).
