CD11c⁺ DCs are infected at low frequencies with HCMV, whereas PDCs are resistant. Purity of PDCs (A) and CD11c⁺ DCs (B) after multiple rounds of positive selection using MACS beads was analyzed by flow cytometry. Results of 1 of 17 representative experiments are shown. Mean purity of 98.98% ± 0.59% and 98.68% ± 1.51% were obtained for PDCs and CD11c⁺ DCs, respectively (n = 17; separate donors). For details, see “Materials and methods.” PDCs and CD11c⁺ DCs were incubated with mock (C, E) or TB40/E (MOI, 10) (D, F) and were cultured for 84 hours. Infection of DCs was detected as cells positive for IEA by intracellular staining and was analyzed by flow cytometry (D, F). Results of 1 of 8 representative experiments are shown (separate donors).