Figure 4.
Inhibition of myeloma cell adhesion to BMSCs by BIBF 1000. Contact cocultures were performed with confluent monolayers of 1 × 105 BMSCs and 2 × 106 CFSE-prelabeled RPMI-8226, U-266, OPM-2, or KMS-11 cells, respectively. Cell cultures were incubated in the absence or in the presence of BIBF 1000 (0.25 to 5.0 μM) for 24 hours. Subsequently, nonadherent cells were washed off 3 times. The remaining adherent cells were trypsinized and quantified by flow cytometry. Data are presented as mean ratios of adherent MM cells over BMSCs from 3 independent experiments for each cell line. Analysis of significance for overall group differences was performed by the Kruskal-Wallis test (U-266, OPM-2, and KMS-11: P = .001; RPMI-8226: NS).
