Figure 2.
Expression of Ezh2 in hematopoietic cells. (A) To isolate the different lineage-negative (Lin-) populations from BM, the 5% most Lin- cells were selected. (B) Cells in the Lin- population were sorted based on Sca-1 and c-kit surface markers. (C) Expression of Ezh2 as measured by qPCR in the different Sca-1 and c-kit populations relative to Sca-1-c-kit- BM cells. (D) Growth of Lin-Sca-1+c-kit+ cells cultured in the presence of GM-CSF and SCF. (E) Relative expression of Ezh2 was monitored by qPCR at different time points after initiation of differentiation with GM-CSF and SCF. Day 0 was set at 1. Insert shows gel analysis of amplified RT-PCR products.

Expression of Ezh2 in hematopoietic cells. (A) To isolate the different lineage-negative (Lin-) populations from BM, the 5% most Lin- cells were selected. (B) Cells in the Lin- population were sorted based on Sca-1 and c-kit surface markers. (C) Expression of Ezh2 as measured by qPCR in the different Sca-1 and c-kit populations relative to Sca-1-c-kit- BM cells. (D) Growth of Lin-Sca-1+c-kit+ cells cultured in the presence of GM-CSF and SCF. (E) Relative expression of Ezh2 was monitored by qPCR at different time points after initiation of differentiation with GM-CSF and SCF. Day 0 was set at 1. Insert shows gel analysis of amplified RT-PCR products.

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