LIGHT-mediated tumor rejection is NK-cell dependent. (A) NK-cell depletion before tumor formation leads to Ag104L^d LIGHT tumor growth. C3B6F1 mice were subcutaneously inoculated with Ag104L^d LIGHT tumor cells through intraperitoneal treatment by anti-asialo GM1 antibody at days -3, 0, and 7 for NK-cell depletion. CD8⁺ cells were depleted with anti-CD8 antibody. Tumor growth was monitored every other day, and data are the mean ± SD of 5 mice for each group. (B) NK-cell depletion results in outgrowth of Ag104 L^d tumor at the secondary tumor challenge. The C3B6F1 mice were subcutaneously inoculated with 1 × 10⁷ Ag104 L^d tumor cells after 40 days of primary Ag104 L^d LIGHT tumor rejection, followed by NK-cell depletion. One of 3 independent experiments and a total of 15 mice per group are presented.