Transplanted human ALDH^hiCD133⁺Lin^- cells differentiate into lymphoid and myeloid progeny in vivo. BM from highly engrafted mice that received transplants of 10⁴ to 105 ALDHhiCD133⁺Lin^- cells was stained with human-specific antibodies for mature hematopoietic lineage markers. (A) Human hematopoietic cells were selected by the expression of human CD45 (R2 = 62.5% ± 10.1%, n = 6) and analyzed for myeloid cell markers CD14 and CD33 (B), B-lymphocyte markers CD20 and CD19 (C), and T-lymphocyte markers CD4 and CD8 (D). Lymphoid and myeloid differentiation was observed after the transplantation of purified ALDH^hiCD133⁺Lin^- cells. T-lymphocyte production was not supported in the NOD/SCID β2M-null or NOD/SCID mouse.