Figure 3.
Detection of human cell repopulation in mice that received transplants of purified ALDHhiCD133-Lin-, ALDHhiCD133+Lin-, or ALDHhiLin- cells. Representative flow cytometric analysis of NOD/SCID β2M-null mice that received transplants of (A-C) 2 × 105 ALDHhiCD133-Lin-, (D-F) 104 ALDHhiCD133+Lin-, or (G-I) 104 ALDHhiLin- cells. At 7 to 8 weeks after transplantation, human hematopoietic cells in the mouse BM were detected by coexpression of CD45 (R1) with CD38 (R2). Cell suspensions from murine spleen and peripheral blood were analyzed in an identical fashion. Injection of more than 2 × 105 ALDHhiCD133-Lin- cells was required to observe human cell engraftment in the BM of NOD/SCID β2M-null mice (n = 3). Mice that received transplants of 104 ALDHhiCD133+Lin- cells showed enhanced engraftment with human cells (18.2% ± 10.9%, n = 6) in the murine BM, compared with mice that received transplants of 104 ALDHhiLin- cells not selected for CD133 expression (5.2% ± 1.7%, n = 5).

Detection of human cell repopulation in mice that received transplants of purified ALDHhiCD133-Lin-, ALDHhiCD133+Lin-, or ALDHhiLin- cells. Representative flow cytometric analysis of NOD/SCID β2M-null mice that received transplants of (A-C) 2 × 105 ALDHhiCD133-Lin-, (D-F) 104 ALDHhiCD133+Lin-, or (G-I) 104 ALDHhiLin- cells. At 7 to 8 weeks after transplantation, human hematopoietic cells in the mouse BM were detected by coexpression of CD45 (R1) with CD38 (R2). Cell suspensions from murine spleen and peripheral blood were analyzed in an identical fashion. Injection of more than 2 × 105 ALDHhiCD133-Lin- cells was required to observe human cell engraftment in the BM of NOD/SCID β2M-null mice (n = 3). Mice that received transplants of 104 ALDHhiCD133+Lin- cells showed enhanced engraftment with human cells (18.2% ± 10.9%, n = 6) in the murine BM, compared with mice that received transplants of 104 ALDHhiLin- cells not selected for CD133 expression (5.2% ± 1.7%, n = 5).

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