The increase in BCL6 mRNA is p53 dependent. (A) The increase in BCL6 mRNA levels after treatment with doxorubicin in lymphoblastoid B cells is attenuated as a result of pretreatment with PFT-α. Lymphoblastoid B cells were subjected to the detailed treatments. BCL6 mRNA levels were determined using QPCR and normalized to β2-microglobulin. Results display mean ± SD of quantifications of 3 independent experiments, each repeated 2 times, each in duplicate. Ctr indicates untreated cells. PFT-α was added at -4 hours; doxorubicin (Doxo) was added at 0 hours; all samples were harvested at +18 hours. □ indicates control. (B) H1299 cells with ts-p53Val135 (ts-p53) or the parental H1299 cells (p53-null) were maintained either at 37°C (p53 inactive) or placed overnight at 32°C (p53 active). BCL6 mRNA levels were determined using QPCR and normalized to β-actin. Fold ratios of mRNA levels were calculated in comparison to that measured in the H1299 cells with ts-p53Val135 maintained at 37°C. Results display mean ± SD of quantifications repeated 3 times, each in triplicate. □ indicates control.
