Doxorubicin induces BCL6 mRNA and protein in lymphoblastoid B cells and mRNA in U2OS cells. (A) (Top) Lymphoblastoid B cells were treated with doxorubicin and harvested at the indicated times. BCL6 mRNA levels were determined using QPCR and normalized to β2-microglobulin. Results display mean ± SD of quantifications repeated 3 times, each in duplicate. Ctr indicates untreated cells. (Bottom) Western blot analysis of BCL6, p53, and β-actin in the lymphoblastoid B cells after the same treatment with doxorubicin. Results displayed are representative of 2 independent experiments. (B) U2OS cells were treated with doxorubicin and harvested at the indicated times. BCL6 mRNA levels were determined using QPCR and normalized to β-actin. p21 served as positive control. Results display mean ± SD of quantifications of 3 independent experiments, each repeated 3 times, each in duplicate. Ctr indicates untreated cells.
