Figure 4.
![Figure 4. IRp60 does not influence eotaxin-induced calcium influx. Eosinophils previously incubated with either anti-IRp60 or control antibodies (isotype, anti-p58.2) followed by the cross-linker sheep anti-mouse F(ab′)2 were washed and loaded with Calcium Green-1AM. The cells were allowed to flow freely in the cytometer until the indicated time point at which eotaxin (1-100 ng/mL) was added (↑). [Ca2+] is expressed as mean fluorescent intensity (MFI). NA indicates not activated; Etx, eotaxin activated (n = 3). Data are expressed as relative cell count ± SD and are representative of 1 of 4 experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/5/10.1182_blood-2005-07-2926/2/zh80050691790004.jpeg?Expires=1722676216&Signature=jqPzJMggjb6vzXi7I4Erpxqj6K24oi4kwybTMFLnK0Xz0D-O47HYlEfUvzONNzHtJsZqorlNdcGUtSzTbQcbwlK-~kaHRTntqyNn64ZCswLe0J72UlxeWgTDw8rsfmkUy4YQwrU11lOAeG0oP~ARWtOZNCWHMv~aA3PvOA8b9DZEpnxuF1lVmQB23roRg5aZ-sx7e~447E8ovxZZgxqSsvpRbFBNpHmtsQZ0lO293qTCATeKoj-FsiXsB~QNR4p9mPR5Jjkcr03RvLnGNbf0AYMurLYLG88CDb6z7hDggI2jV9ccSYYRkk4nhvt4zHgFFP1yR3LP8XZh3dga9NDoCQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IRp60 does not influence eotaxin-induced calcium influx. Eosinophils previously incubated with either anti-IRp60 or control antibodies (isotype, anti-p58.2) followed by the cross-linker sheep anti-mouse F(ab′)2 were washed and loaded with Calcium Green-1AM. The cells were allowed to flow freely in the cytometer until the indicated time point at which eotaxin (1-100 ng/mL) was added (↑). [Ca2+] is expressed as mean fluorescent intensity (MFI). NA indicates not activated; Etx, eotaxin activated (n = 3). Data are expressed as relative cell count ± SD and are representative of 1 of 4 experiments.
