Cell cycle analyses of Ba/F3 KIT-D816V and KIT-WT cells after inhibitor treatment. (A) Ba/F3 cells expressing KIT-WT and KIT-D816V were grown for 24 hours in presence or absence of the indicated doses of PKC412 and imatinib and subsequently analyzed by propidium iodide (PI) staining of cell nuclei. Sub-G1 nuclei that had lost parts of their DNA by fragmentation were defined as apoptotic. KIT-WT cells were cultured in the presence of 100 ng/mL SCF. All values represent means and standard deviations from 3 independent experiments. (B) KIT-D816V cells treated with the indicated concentrations of PKC412 and imatinib were analyzed for cell cycle distribution. All values represent means and SDs from 3 independent experiments. (C) Representative histograms from flow cytometric analysis of PI-stained nuclei of Ba/F3 cells expressing KIT-D816V.
