Progenitor-derived LCs persist in the skin. Epidermal cells were isolated from ears of chimeric mice 3 months after progenitor transfer. (A) Histograms in left panel show CD45.1 expression profile of total epidermal cells derived from CD45.2 mice reconstituted with either 10⁴ CD45.1 CMPs, 4 × 10⁴ GMPs, or 10⁴ CLPs. Contour plots show CD11c/I-A^b expression profile of gated donor (CD45.1⁺) or host-derived (CD45.1^-) epidermal cells. Percentages of LCs derived from transplanted cells (CD45.1⁺) or host cells (CD45.1^-) are indicated. (B) Chart shows average LC reconstitution (% of total LCs) obtained per 10⁴ progenitor cells at 3 months after adoptive transfer. Data are representative of at least 3 transplantations per progenitor population with similar results.