Phenotype of lineage-negative cells from catalase-treated LTBMCs. Nonadherent cells removed from culture at week 2 were stained with an antilineage cocktail (FITC) and anti-c-Kit (PE), Sca-1 (APC), FcγR (PE-Cy7), and IL7Rα (biotin/streptavidin-tricolor). Representative histograms are shown but similar results have been obtained repeatedly. (A) Histogram showing the staining with the lineage cocktail in the control and catalase-treated cultures. The region labeled LIN^- was then used as a gate to analyze the distribution of the other markers. (B) LIN^- cells from catalase-treated cultures were further analyzed for their expression of Sca-1 (x-axis in all 2-dimensional histograms) and for the expression of c-Kit, FcγR, and IL7Rα. (C) LIN^- cells from control cultures analyzed as in panel B.