Figure 5.
![Figure 5. Autologous T-cell proliferation by DCs generated in the presence of TPO and TNF-α. The cells prepared by culture for 7 days with 100 ng/mL TNF-α in the presence (▪) or absence (□) of 100 ng/mL TPO were used at graded doses as stimulator cells after γ-irradiation. In some MLRs, anti-CD3 mAb was added. Proliferation was assessed by adding 3H-thymidine (1 μCi per well [37 κBq/well]) during 72 to 90 hours of culture. Results represent the mean ± SD of triplicate cultures.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/4/10.1182_blood-2005-08-3155/2/zh80040691350005.jpeg?Expires=1723389517&Signature=xwOp-BSbtqrq2ht6gGUsL6TWMTJaLXOGlhEUhdiwGuTU3VSOwoZZSOSS3Gme4eWN7-U0SDJQsf7CuNwhek0MDHBCsdZe~jXTehHzsvEjiHoL1FM4~z0bMBSdqzuVnIsk0O4tgj-FFxoIxyY1b2eCL7nDhAiOyYwXO~uXCvCVmvk1sOGbaOUe~FpRgMT5DTkXjSLHLwjoez77qm8Kx5k81eHFRUheuXty1lwIKaNyj0AuWl6vCmkv4IBYqtnkDTGDl14Faz63OhrcdxX9HMvWeUylzVNR9UH0qTBMpI5IOqq9qE8ngvttxMzU7Wyu5cRhsiUXV96CwyqsFfQCaUPvDw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Autologous T-cell proliferation by DCs generated in the presence of TPO and TNF-α. The cells prepared by culture for 7 days with 100 ng/mL TNF-α in the presence (▪) or absence (□) of 100 ng/mL TPO were used at graded doses as stimulator cells after γ-irradiation. In some MLRs, anti-CD3 mAb was added. Proliferation was assessed by adding 3H-thymidine (1 μCi per well [37 κBq/well]) during 72 to 90 hours of culture. Results represent the mean ± SD of triplicate cultures.
