Dose dependence of the effect of exposure to AT-2 HIV. DCs were cocultured with autologous CD4⁺ T cells as described in Figure 1. T cells had been activated in the presence of either control microvesicles (vesicles) or graded doses of AT-2 HIV (0.125-1 μg equiv^p24/10⁶ cells). (A) Inhibition of CD86 expression in one representative subject. CD86 was determined on DCs, gated on forward-scatter and CD1a expression. Numbers in the graph represent the MFI for each condition. (B) Summary of the inhibition observed in 4 subjects. Results are shown as the mean ± SE of the results obtained in 4 individual subjects. Numbers in the graph represent the P value for each comparison (ANOVA).