American Society of Hematology

Figure 7.

$Figure 7. CD83L engagement supports sustained growth of antigen-specific CD8+ T cells. MART1-specific CD8+ T cells were generated by stimulation with MART1-pulsed APC/A2/CD80/CD83 every 7 to 14 days. The percentage increase or decrease in the number of cells was determined at each stimulation, and a fraction was subsequently stimulated and maintained in culture. Antigen specificity was demonstrated every 3 or 4 rounds of stimulation by tetramer analysis. (A) The predicted total number of CD8+ T cells generated over a 191-day culture period is shown. Arrow denotes removal of debris by Ficoll density gradient. (B) PE-conjugated MART1 tetramer staining versus CD8 staining is shown at 4 time points during the culture period. (C) Effector function was determined by cytotoxicity assay on day 190 using peptide-pulsed T2 cells as targets (▪ indicates MART1 peptide; •, HIV pol as control peptide).$

CD83L engagement supports sustained growth of antigen-specific CD8⁺ T cells. MART1-specific CD8⁺ T cells were generated by stimulation with MART1-pulsed APC/A2/CD80/CD83 every 7 to 14 days. The percentage increase or decrease in the number of cells was determined at each stimulation, and a fraction was subsequently stimulated and maintained in culture. Antigen specificity was demonstrated every 3 or 4 rounds of stimulation by tetramer analysis. (A) The predicted total number of CD8⁺ T cells generated over a 191-day culture period is shown. Arrow denotes removal of debris by Ficoll density gradient. (B) PE-conjugated MART1 tetramer staining versus CD8 staining is shown at 4 time points during the culture period. (C) Effector function was determined by cytotoxicity assay on day 190 using peptide-pulsed T2 cells as targets (▪ indicates MART1 peptide; •, HIV pol as control peptide).

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