Effector function of MART1-specific CD8⁺ T cells. (A) MART1-specific cytotoxic CD8⁺ T cells generated by APC/A2/CD80/CD83 killed target cells in an HLA-A2-specific manner. Target cells (T2 and Jurkat cells transduced with vector or A2) were pulsed with MART1 peptide (▪) or control peptide (•). ¹¹LLFGYPVYV¹⁹ peptide of the TAX of HTLV-I was used as a negative control peptide. (B) Blocking experiments were performed using HLA-A2-specific mAb (BB7.2) and isotype control (mIgG2b). Peptide-pulsed T2 cells were used at an E/T ratio of 30:1. (C) MART1-specific cytotoxic CD8⁺ T cells generated by APC/A2/C80/CD83 secreted IFN-γ in an antigen-specific manner. Error bars represent standard deviation (SD).