Figure 7.
Figure 7. Spatiotemporal analysis of alloreactive T cells and tumor cells by bioluminescence imaging after allogeneic BMT. Lethally irradiated (1300cGy) B6D2F1 recipients underwent transplantation with 5 × 106 WT TCD BM and either 0.5 × 106 WT or β7-/- splenic T cells. P815 tumor cells (0.5 × 106) that had been transduced with an LTR-HSV1 TK-EGFP-Luc retroviral vector were infused into each mouse at the time of transplantation. (A) Mice were tracked for in vivo luminescence 10 minutes after intraperitoneal injection of firefly luciferin. (B) Average luminescence, quantified as photons per sec/m2 at time points with all mice still alive, demonstrates delay in tumor growth in recipients of β7-/- T cells (P = .08).

Spatiotemporal analysis of alloreactive T cells and tumor cells by bioluminescence imaging after allogeneic BMT. Lethally irradiated (1300cGy) B6D2F1 recipients underwent transplantation with 5 × 106 WT TCD BM and either 0.5 × 106 WT or β7-/- splenic T cells. P815 tumor cells (0.5 × 106) that had been transduced with an LTR-HSV1 TK-EGFP-Luc retroviral vector were infused into each mouse at the time of transplantation. (A) Mice were tracked for in vivo luminescence 10 minutes after intraperitoneal injection of firefly luciferin. (B) Average luminescence, quantified as photons per sec/m2 at time points with all mice still alive, demonstrates delay in tumor growth in recipients of β7-/- T cells (P = .08).

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