WT and β₇^-/- T cells do not differ in activation or alloreactive proliferation and have intact cytotoxicity. WT and β₇^-/- donor T cells were labeled with CFSE and were injected intravenously into sublethally irradiated (750 cGy) B10.BR recipients. Donor splenic T cells from recipient mice were analyzed 72 hours after infusion. (A) Histogram overlay of dividing donor CD4⁺ and CD8⁺ CFSE-labeled T cells (shaded area, β₇^-/-; outline, WT) show nearly identical proliferation kinetics. (B) CD44, CD62L, and CD25 expression on CD4⁺ and CD8⁺ donor T cells, with percentage positive T cells expressed as a percentage of fast proliferating T cells (outline = β₇^-/-; shaded area = WT). (C) Lethally irradiated (1300 cGy) B10.BR recipients underwent transplantation with 5 × 10⁶ WT TCD BM and either 1 × 10⁶ WT or β₇^-/- splenic T cells, and spleens were analyzed at day 11 after HSCT by flow cytometry. Graph represents the percentage of donor-derived CD4⁺CD25⁺FoxP3⁺ T cells (P = NS). (D) Mice underwent transplantation and harvest as in panel C. Percentage of donor CD4⁺CD44^hiCD62L^lo (effector) and CD4⁺CD44^hiCD62L^hi (central memory) populations. (E) Percentage of donor CD8⁺CD62L^lo population. (F) Percentage of donor CD8⁺CD44^hi population. (G) Splenocytes from B6D2F1 HSCT recipients of WT versus β₇^-/- T cells were analyzed at day 14 after HSCT against syngeneic (EL4) and allogeneic (P815) target cells (n = 5).