Impaired multilineage HSC repopulating ability in p85α^-/-; p85β^+/- fetal liver cells. (A) Mean donor chimerism 1, 4, and 6 months following transplantation. ^*P < .01 compared with WT; ^**P < .05. (B) Representative dot plots from multilineage analyses. The percentage above each dot plot represents the calculated donor contribution to an individual lineage. Donor contribution to individual lineages was calculated by dividing CD45.2⁺lineage⁺ by total lineage⁺ cells (UR/[UR+LR]). (C) Donor contribution to myeloid and lymphoid lineages in long-term reconstituted mice. ^*P < .01 compared with WT; ^**P < .05. Error bars represent the standard error of the mean.