CLA⁺ CD43 displays E-selectin ligand activity in an antigen capture flow assay. Anti-CD43 mAb 1G10 (left) and isotype control Ab (right) bound to plastic were overlayed with lysates of cultured T cells. CHO-E cells (▪), but not CHO-P () or mock-transfected cells (), tethered and rolled on CD43 adsorbed from lysates of CLA⁺ T cells. No significant adhesion was observed on CD43 adsorbed from lysates of CLA^- T cells or on isotype control substrates. CHO-E binding to CLA⁺ CD43 was abrogated in the presence of EDTA (□). Results shown are mean and SEM of 3 to 6 separate determinations on each substrate collected in 2 independent experiments.