Reducing and nonreducing SDS-PAGE conditions result in different CLA band patterns on Western blots. Aliquots of CLA⁺-cultured T-cell lysate were subjected to SDS-PAGE under reducing (left panel) and nonreducing (right panel) conditions, transferred to PVDF membranes, and immunostained for CLA (mAb HECA-452). Three CLA⁺ bands (240 kDa, 140 kDa, and 125 kDa) are observed on Western blots following nonreducing SDS-PAGE, as opposed to one band (140 kDa) after reducing SDS-PAGE. High-range molecular weight markers are run in the flanking lanes as shown. Molecular weight values in kDa are shown beside each blot.