Synergy of IFN-γ induction by ITAM-bearing receptors. Liver lymphocyte receptor cross-linking was performed with anti-Ly49H (3D10) (A-B), anti-NKG2D (3A10) (C-D), and anti-CD3 (500A2) (E-F). IFN-γ mRNA (A,C,E) and protein (B,D,F) expression were evaluated at 1.5, 3, and 5 hours. RPA evaluation of IFN-γ mRNA was normalized to control GAPDH. Values are representative of 3 experiments.