Differences in mRNA expression in peptide-specific T cells before and after ASCT. (A) PBMCs of a DR4 heterozygotic patient, from time points before (full-blown disease) and 2 year after ASCT (complete remission) and (B) from a DR4 heterozygotic patient, from time points 5 years after ASCT (patient still in remission) and 7 years after ASCT (complete relapse of disease) were stimulated with a peptide derived of human hsp60. After 4 days, peptide-specific CD4⁺ T cells were captured and sorted, using the indicated gates. CTB-FITC and CD4-CY were used to visualize CD4⁺ T cells bound by the aAPCs. Liposomes containing complete rafts but no MHC-peptide complexes were used as negative controls. The sorted peptide-specific CD4⁺ T cells were lysed and analyzed on the mRNA expression of the transcription factors T-bet and GATA-3 and the cytokines IL-10 and IFN-γ by quantitative PCR.