High expression of mRNA FoxP3 in thymus-derived CD4⁺CD25⁺ T cells. CD4⁺CD25^-, CD4⁺CD25⁺CD45RO, and CD4⁺CD25⁺CD45RA T cells were isolated from the PB of 3 systemic JIA patients on conventional therapy and from 3 systemic JIA patients at different time points after ASCT by FACS sorting. mRNA FoxP3 was measured by quantitative PCR. Data are expressed as the mean normalized gene expression (± SEM) of the 3 patients.