LIME is required for BCR-dependent NF-κB translocation. (A) LIME mediates BCR-dependent NF-κB translocation to the nucleus. A20 cells were transfected with pLL3.7 plasmids or pLL3.7 expressing siRNA against LIME. After 48 hours, transfected cells were activated with 20 μg/mL F(ab′)₂ goat anti-mouse IgG for 3 hours at 37°C. The cells were fixed and permeabilized with 0.1% Triton X-100, after which the localization of NF-κB was detected by an anti-p65 Ab and Alexa 568-conjugated anti-rabbit IgG. (B) Graph shows the number of cells with nuclear translocation of NF-κB.