Figure 1.
LIME expression in primary mouse splenic B cells. (A) Splenic B cells were isolated from C57BL/6 mice using anti-B220 (CD45R) microbeads. Cells were activated by F(ab′)2 goat anti-mouse IgM for 0, 3, 6, 12, 18, or 24 hours and then lysed. Lysates were analyzed by immunoblotting with mouse polyclonal anti-LIME or antiactin Abs. (B) Splenic T cells were isolated from C57BL/6 mice using anti-Thy1.2 microbeads. T cells were activated by anti-CD3 and CD28 Ab. B cells were purified and activated as described in panel A. Lysates were analyzed by immunoblotting with rabbit polyclonal anti-LIME or antiactin Abs.
