American Society of Hematology

Figure 3.

$Cytochrome c release from the mitochondria and caspase 3 activity on treatment with GA. (A) RBL cells were exposed to increasing concentrations of GA (0, 0.5, 1 μM). Mitochondria were isolated, and cytochrome c was detected in the cytosolic and mitochondrial fractions by anti–cytochrome c antibody, as described in “Materials and methods.” Results of 1 of 3 representative experiments are shown. (B) RBL cells were exposed to increasing concentrations of GA (0, 0.5, 1 μM). Caspase 3 activity was determined by colorimetric product with absorbance at 405 nm. Results of 1 of 3 representative experiments are shown. (C) RBL cells were grown in 96 wells and exposed to increasing concentrations of GA (0, 0.25, 0.5, 0.75, 1 μM) for 15 hours. Cell viability was assessed by cell-viability assay, as described in “Materials and methods.” Data are represented as mean ± SE (n = 4). Results of 1 of 3 representative experiments are shown.$

Cytochrome c release from the mitochondria and caspase 3 activity on treatment with GA. (A) RBL cells were exposed to increasing concentrations of GA (0, 0.5, 1 μM). Mitochondria were isolated, and cytochrome c was detected in the cytosolic and mitochondrial fractions by anti–cytochrome c antibody, as described in “Materials and methods.” Results of 1 of 3 representative experiments are shown. (B) RBL cells were exposed to increasing concentrations of GA (0, 0.5, 1 μM). Caspase 3 activity was determined by colorimetric product with absorbance at 405 nm. Results of 1 of 3 representative experiments are shown. (C) RBL cells were grown in 96 wells and exposed to increasing concentrations of GA (0, 0.25, 0.5, 0.75, 1 μM) for 15 hours. Cell viability was assessed by cell-viability assay, as described in “Materials and methods.” Data are represented as mean ± SE (n = 4). Results of 1 of 3 representative experiments are shown.

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