Regulation of DC cytokine expression in the presence of a DCAL-2 signal. iDCs were stimulated with or without indicated doses of LPS or Zymosan in the presence or absence of anti-DCAL-2 (10 μg/mL) or IgM control (10 μg/mL). After 24 hours, supernatants were harvested and the levels of cytokines or chemokines analyzed by ELISA. (A) Induction of cytokines and chemokines by anti-DCAL-2 stimulation. (B) DCAL-2 signal modulated LPS- or zymosan-induced IL-12p40 and p70 expression. Results are representative of 4 independent experiments. Mean of triplicate wells ± SEM. ^*P < .05; ^**P < .01, paired Student t test.