DCAL-2 ligation induces CCR7 expression on iDCs and TLR-stimulated DCs. iDCs were stimulated with or without LPS (1 μg/mL) or zymosan (100 μg/mL) in the presence or absence of anti-DCAL-2 (10 μg/mL) or IgM control (10 μg/mL). After 24 hours, cells were harvested and washed, then double stained with (A) anti-CD86 and anti-CCR7 or (B) anti-CD83 and anti-DC-LAMP as described in “Materials and methods.” Results are representative of 4 independent experiments.