The mRNA and protein expression of hDCAL-2 is restricted to myeloid cells. (A) RT-PCR analysis of hDCAL-2 in a panel of primary cells shows that hDCAL-2 is expressed in human CD14⁺ monocytes, human iDCs, CD1a⁺ DCs, and macrophages but not in NK cells, PDCs, B cells, activated B cells (activated by IgM and anti-CD40 mAb for 24 hours), T cells, or activated T cells (activated by CD3 mAb). Results are representative of 3 different donors. (B) Fluorescence-activated cell sorter (FACS) analysis of hDCAL-2 in a panel of PBMCs shows that hDCAL-2 is expressed on human CD1a⁺ DCs but not on B cells, T cells, NK cells, or pDCs. Results are representative of 3 different donors. (C) FACS analysis of human primary cells and cell lines shows hDCAL-2 is expressed strongly on CD14⁺ monocytes and iDCs but only weakly on the HL-60, U937, and Nalm-6 cell lines. Results are representative of 3 independent experiments.