Human DCAL2 gene structure and sequence. (A) The nucleotide and protein sequence of hDCAL-2. The ITIM in the cytoplasmic tail is outlined and indicated in bold; the putative transmembrane region is underlined. The 6 cysteine residues that create 3 disulphide bonds in the CRD are circled and shaded, and 4 potential glycosylation sites are boxed. (B) Alignment of the hDCAL-2 CRD protein sequence with related CLRs. ^*Conserved cysteine residues; two dots, a conserved charge residues. The WIGL domain is shown in bold. (C) A phylogenetic tree of the CLRs most closely related to hDCAL-2.