Apoptosis-related gene expression profile induced by MDM2 antagonists. Cells from B-CLL patients with wild-type p53 (patients 20, 3, and 15; □) and mutant p53 (patients 16 and 17; ▪) were untreated or treated with 5 μM nutlin-3a for 48 hours. Cells were lysed, and expression of apoptosis-related genes was analyzed by RT-MLPA as described in “Patients, materials, and methods.” The results are shown as fold induction relative to untreated cells.