Figure 2.
Immunoblotting of pellets and releasates in activated platelets. Platelets were incubated with 1 U/mL thrombin for 0, 30, 60, or 120 seconds. Following termination, platelet pellet and releasates were prepared, separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, and immunoblotted with anti-PrPC Ab 308 (A), and densitometry was performed (B) on pellet (□) and releasates (▦) (n = 3). Error bars indicate standard error of the mean.
