Adhesion of Jurkat cells expressing WT and the S245A CXCR3 to VCAM-1 and FN in response to CXCL11. (A) Denatured and intact CXCL11 induced adhesion of Jurkat cells, expressing WT or the S245A CXCR3, to immobilized recombinant human (rh) VCAM-1 are shown. Adhesion of cells is monitored over a period of 1 minute while detachment forces (dyn/cm2) continuously increased. (B) Denatured and intact CXCL11-induced adhesion of Jurkat cells, expressing WT or the S245A CXCR3, to immobilized fibronectin are shown. Adhesion of cells is monitored over a period of 1 minute while detachment forces (dyn/cm2) continuously increased. (C) CXCL11-induced adhesion of Jurkat cells, expressing WT or the S245A CXCR3, to immobilized rhVCAM-1 5 seconds after the detachment force was increased to 5 dyn/cm2.
