![Figure 2. Cell-surface expression of WT and mutated CXCR3 receptors on HEK 293 cells. WT and mutant CXCR3 cell-surface expression on stable transfected HEK 293 cell clones was tested by immunostaining with anti-CXCR3 specific antibodies and analyzed by FACS (black line). Immunostaining with IgG1 isotype control antibodies is in gray line. (A) WT CXCR3. (B) 349stopΔ20 C-tail truncated. (C) 332stopΔ37 C-tail truncated. (D) 332-334L→A C-tail mutations. (E) S245 to A 3i loop mutation. (F) Double-mutant of 332stopΔ37 C-tail truncated and S245 to A 3i loop mutation. (G) HEK 293 cells. Counts indicate relative cell number. A representative histogram of at least 3 experiments performed is presented. (H) Binding of biotinylated I-TAC (mean florescence intensity [MFI]) at different concentrations (nM) to 293 cells expressing the different mutated or truncated CXCR3.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/10/10.1182_blood-2004-01-0214/2/zh80100695830002.jpeg?Expires=1722425754&Signature=VuYltep-ucPRgMQn58B6oQXFges5ZEFvZl~uLhJQe-0PrZDwA28fI5ZQ3GFchA4uySSVG2nxcueVfaXR04mdrA-SvguvvdSrGHiFLTZ~owjnhykL1nsYcZRDLDNgLvXoj7aHdtt5brwEvBsxdahDjqsiMlF2typ78V-gGJXxVsChJ6MVjo6jtGazcZUpkdLizJnRQiUatbHFvUrWpMCn6fN5aFGp4zLIWDaXPLry~VBQgQkC3d26-lvMktt2c~ZayQeF-9SWyBLOQ~xHm~wH5PuktXGfPXThq67h3Ap4ZgS0YXIWJ3IMfrNr1Neeyl2ZjK1Fqt6tqdH-kZmmLlB0zA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cell-surface expression of WT and mutated CXCR3 receptors on HEK 293 cells. WT and mutant CXCR3 cell-surface expression on stable transfected HEK 293 cell clones was tested by immunostaining with anti-CXCR3 specific antibodies and analyzed by FACS (black line). Immunostaining with IgG1 isotype control antibodies is in gray line. (A) WT CXCR3. (B) 349stopΔ20 C-tail truncated. (C) 332stopΔ37 C-tail truncated. (D) 332-334L→A C-tail mutations. (E) S245 to A 3i loop mutation. (F) Double-mutant of 332stopΔ37 C-tail truncated and S245 to A 3i loop mutation. (G) HEK 293 cells. Counts indicate relative cell number. A representative histogram of at least 3 experiments performed is presented. (H) Binding of biotinylated I-TAC (mean florescence intensity [MFI]) at different concentrations (nM) to 293 cells expressing the different mutated or truncated CXCR3.
